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Transcriptional analysis of abdominal fat accretion in genetically fat and lean chickens: A new polygenic model of visceral obesity
Author(s) -
Cogburn Larry Albert,
Resnyk Christopher,
Porter Tom E.,
Aggrey Sam E.,
Le BihanDuval Elisabeth,
Duclos Michel,
Simon Jean
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.862.7
Subject(s) - endocrinology , medicine , adipokine , adipose tissue , biology , lipogenesis , transcriptome , obesity , lipid metabolism , leptin , peroxisome proliferator activated receptor gamma , genotype , type 2 diabetes , gene , genetics , gene expression , diabetes mellitus , peroxisome proliferator activated receptor
The purpose of this study was to characterize the adipose transcriptome in experimental chickens divergently selected over many generations for a 3‐ to 4‐fold difference in abdominal fatness at the same body weight and feed intake. Our time‐course transcriptional analysis of abdominal fat in juvenile fat (FL) and lean (LL) chickens revealed 354 differentially expressed (DE) genes as a main effect of genotype and 254 DE genes in the genotype by age interaction. The LL chickens showed higher expression of a large number of genes involved in hemostasis ( F2, F9, FGA, PLG, A2M, PROC, CFB, SERPINA4, SERPIND1 and SERPINE1 ), retinol metabolism/action (BCMO1, RARRES2, RBP4, RBP7, RDH1, NOL7 ), thyroid hormone metabolism ( DIO1 and DIO3 ) and angiogenesis ( ANG and ANPTL3 ). Several genes involved in lipogenesis were expressed at higher levels in FL adipose tissue after 5 weeks of age [ THRSPA , TAS1R1, SCD1, PPARG , and IKBA and visfatin ( NAMPT )]. Apparently, genetically fat chickens exhibit many of the same co‐morbidities as obese humans (i.e., insulin resistance, diabetes and cardiovascular disease), presumably caused by dysregulation of endocrine/adipokine signaling and energy expenditure across multiple tissues. This work was supported the USDA‐IFAFS Animal Genome Program (Grant 00‐52100‐9614).