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A molecular signature of resistance training‐induced human skeletal muscle hypertrophy
Author(s) -
ThalackerMercer Anna,
Merritt Edward,
Cui Xiangqin,
Bamman Marcas
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.862.6
Subject(s) - muscle hypertrophy , skeletal muscle , myocyte , microarray , phenotype , biology , gene expression , gene , gene expression profiling , microarray analysis techniques , microbiology and biotechnology , endocrinology , genetics
Using K‐means cluster analysis, we have previously shown that human adults have widely divergent myofiber growth responses to lower body progressive resistance training (PRT) with some having no response (NON), a modest response (1,111μm 2 mean myofiber growth, MOD), or an extreme hypertrophic response (2,475μm 2 , XTR) PMID: 17395765. Underlying mechanisms for this differential growth response are largely unknown. The purpose of this study is therefore, to determine whether a molecular signature exists, prior to 16 weeks of PRT, which could predetermine the skeletal muscle hypertrophic response in adults. mRNA was isolated from muscle biopsies taken from the vastus lateralis of 44 previously clustered men and women (aged 20–75y). Agilent 4X44K single color genechips were used to determine differences in gene expression among the three clusters and identify informative genes which could predict the myofiber response phenotype to PRT. Microarray results were followed‐up with qRT‐PCR and immunoblotting. By comparing XTR to NON, our results suggest distinctive molecular signaling networks that may underlie the individual differences in potential for mechanically‐induced myofiber hypertrophy. Support: NIH 1R01 AG017896 , VA Merit Review