High brown adipose tissue thermogenic parameters in adult female of dams fed low salt diet during pregnancy

Objective To evaluate the energy metabolism and estrogen influence in adult female offspring whose mother received salt restriction during pregnancy in brown adipose tissue (BAT) Methods Female Wistar rats were fed low (LS: 0.15%) or normal (NS 1.3% NaCl) salt diet from 8 to 18 weeks of age. At 12 weeks of age, they were mated. Body weight (BW) was measured on the day of birth and all off groups were fed NS since weaning. With 40 days of age, offspring were ovariectomized (OVX) or not (CON). Nor epinephrine (NOR), epinephrine (EPI), leptin (LEP) and the coenzimes (COQ9 and COQ10), and gene expression of proteins related with energetic metabolism such as uncoupling protein‐1 (UCP1), coenzymes (COQ1 and COQ2), PPAR γ and PGC1 α were measured at 12‐week ‐age. Results (mean±SEM, p<0.05, n=5/group) BW (g) was lower at the birth in LS (5.1±0.2) than in NS (5.9 ±0.1). The NOR (pg/dL) was higher in NS OVX (403±7) than in NS CON (258±6). The EPI (pg/dL), was higher in NS OVX (983±204) than NS CON (393±68), but the NOR and the EPI were not different among LS groups. LEP (mg/dL) was higher in both OVX groups. The coenzimes COQ9 and COQ10 (pg/dL) were higher in LS OVX (2.7±0.3) than LS CON (1.8±0.3) and LS OVX (0.2±0.01) than LS CON (0.05±0.001). All termogenic parameters in LS OVX were higher than in NS group. Conclusions Low salt diet during pregnancy and lactation causes enhanced energy metabolism in OVX adult female offspring. Supported by FAPESP.