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The effect of exercise on NF‐κB activation in vivo is independent of diet
Author(s) -
Heckstall Ebony Monique,
Lloyd Jesse,
Ogaye Koma,
Moffat Jennifer,
Keslacy Stefan
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.858.17
Purpose investigate the kinetic of NF‐kB expression in vivo following high fat diet (HFD) and exercise. Methods NF‐kB Tg mice were exposed to HFD and trained for 12 weeks. NF‐kB activity was imaged in vivo using an IVIS. Results HFD mice showed a weight gain of 25%, corresponding to increased NFkB luciferase for the whole body (P<0.05) and 58% increase in the belly when compared to normal diet (ND). Following acute exercise NF‐kB activation increased in the ND and decreased in the lymph nodes. Both groups demonstrated a similar NFkB activity downregulation following chronic exercise. We found a significant correlation between NF‐kB activation and weight in HFD mice. P65 expression was identical in both groups and in all tissues. No changes were found in the insulin receptor, but its phosphorylation level decreased in the liver of HFD mice (P<0.05) Conclusion We demonstrated that HFD induced NF‐kB activation in vivo , corresponding to a decreased insulin signaling. Acute exercise enhanced NF‐kB activation in ND mice and chronic exercise downregulated NF‐kB similarly in both groups. Surprisingly, fat was not the source of NF‐kB activation.