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Single cell analysis of voltage‐gated potassium channels for electrophysiological properties of rat hypothalamic paraventricular nucleus neurons
Author(s) -
Lee Seul Ki,
Lee Sora,
Shin Seung Yub,
Ryu Pan Dong,
Lee So Yeong
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.850.3
The hypothalamic paraventricular nucleus (PVN), an integrating site in regulating the neuroendocrine and autonomic nervous systems, is composed of heterogeneous neurons, type I and type II, based on their electrophysiological properties. In the present study, the molecular identification of voltage‐gated potassium (Kv) channels was investigated that are relative with a delay in the onset of the first action potential present distinctively in type I neurons. The recorded cells were harvested and followed by single cell RT‐PCR and real‐time PCR analysis. As a result, Kv1.2, Kv1.3, Kv1.4, Kv4.1, Kv4.2 and Kv4.3 were expressed in both type I and type II neurons and the expression density of Kv4.2 and Kv4.3 were significantly higher in type I neurons than in type II. Additionally, co‐expression of Kv1.3, Kv4.2 and Kv4.3 prolonged delay significantly and Kv channel blockers diminished the delaying time in the onset of the first action potential in type I PVN neurons. In conclusion, Kv1.3, Kv4.2 and Kv4.3 are the potential Kv channels responsible for determining the distinct electrophysiological properties of PVN neurons. Support from the National Research Foundation of Korea (2010‐0015531).