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Association of Bone Strength with Plasma Leptin in Rats After Severe Burn and Disuse
Author(s) -
Baer Lisa A,
Wu Xiaowu,
Tou Janet,
Wolf Steven E,
Wade Charles E
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.849.7
Circulating leptin, a regulator of energy stores and body composition, is related to bone mass. Burn with hindlimb unloading (HU) results in substantial and sustained bone loss in rodents. Using this model we observed decreased bone mineral content and strength. We sought to demonstrate an association between leptin concentration and the effect on bone strength after injury and disuse. Male rats were randomized into four groups: Sham Ambulatory (SA;n=10), Burn Ambulatory (BA;n=9), Sham HU (SH;n=10) or Burn HU (BH;n=10). Burn rats received a 40% total body surface area scald burn and immediately hindlimb unloaded. 14 days after injury femur weight was measured and blood collected for leptin levels. Three‐point bending testing of femurs biomechanical strength was completed. Significant reductions were observed in femur weight in both unloading groups as compared to ambulatory groups. Leptin levels were significantly reduced in BA, SH and BH from SA, but BH showed the most dramatic decrease. Both bending failure and ultimate bending, indicators of bone strength, were significantly reduced in BA and BH from SA. Bending failure was significantly correlated with leptin (r=0.57; p<0.02), as well as ultimate bending (r=0.44; p<0.02). Burn and disuse causes metabolic changes that may have a long term effects on bone strength. (US Army MRMC & Juvenile Diabetes Foundation