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Effects of ovariectomy on the cardiovascular system and ANP production in FVB/N Mas knockout mice
Author(s) -
Machado Fernanda B.,
Santos Robson A. S.,
Bonfa Paula,
Lautner Roberto,
Melo Marcos,
Bader Michael,
Reis Adelina
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.848.7
The vasodilator Ang‐1–7/Mas axis, new component of the renin‐angiotensin system (RAS), have been described in different tissues and have an important contribution for the blood pressure (BP) regulation. Higher BP and endothelial dysfunction were observed in male mice knockout (KO) for Mas receptor. These characteristics are also common among postmenopausal women that, in addition, have an impaired natriuretic peptide system. This study assessed the influence of ovariectomy (ovx) on cardiovascular system and ANP production of FVB/N Mas‐KO. KO systolic BP, measured by plethysmography, was lower than in wild type (WT) (107±2 vs. 118±5 mmHg, p<0.05) and it was not altered by ovx. Vasoconstriction response to phenylephrine in aortic rings of KO was higher than WT (Emax: 2.5±0.5 vs. 1.3±0.2mN/mm p<0.05) and this difference was reversed after ovx. The vasodilator response to ACh was lower in KO than in WT (Emax: 37.6±4.4 vs. 83.6±4.0%; p<0.05). Upon ovx, this response was markedly reduced in WT but not in KO. Plasma ANP measured by RIA was lower in ovariectomized KO than in WT (82±6 vs. 132±12 pg/mL, p<0.05). Echocardiography revealed a significantly decreased fractional shortening on hearts of KO (35±1 vs. 39±1%; p<0.05), indicating lower global ventricular function. Our Data suggest that Mas deletion mediates imbalance of the cardiovascular system similar to one evoked by ovx. Support: CNPq/Capes