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Upregulation of vasopressin V1a and V2 receptors in the PVN of rats modulates the cardiovascular response to air‐jet stress
Author(s) -
Lozic Maja,
Greenwood Michael,
Sarenac Olivera,
Zigon Nina Japundzic,
Murphy David
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.845.15
This paper investigates the role of PVN vasopressin receptors in the cardiovascular response of Wistar radio‐telemetred rats to 10‐minute‐long air‐jet stress. Rats were microinjected with replication‐deficient adenoviral vectors carrying genes for V1a receptors (Ad.CMV.eGFP plus Ad.CMV.V1a; n=4), V2 receptors (Ad.CMV.eGFP plus Ad.CMV.V2; n=6) and green fluorescent protein (Ad.CMV.eGFP, n=6) for control. Only rats in which gene expression was observed within the PVN post mortem, were subjects of cardiovascular data analysis. Autonomic cardiovascular regulation was evaluated using spectral analysis and the baroreflex functioning using the method of sequences. The results show that basal values of cardiovascular parameters of V1a‐ and V2‐transfected rats did not differ in respect to controls. Although exposure to stress increased SBP, DBP, LF‐SBP and HF‐SBP of all rats, only V1a‐ and V2‐transfected rats exhibited LF‐HR and HF‐HR increase. In V1a‐overexperssing rats LF/HF‐HR increased while in V2‐transfected rats the LF/HF‐HR decreased. Exposure to stress decreased baroreflex sensitivity in controls but not in V1a‐ and V2‐transfected rats. The results indicate that upregulation of V1a and V2 receptors in PVN modulates differentially the cardiovascular response to stress. The source of research support: Royal Society UK, Ministry od Science (RS)