Premium
Brain derived neurotrophic factor (BDNF) in the nucleus tractus solitarii (nTS) contributes to sympathoexcitation in spontaneously hypertensive rats
Author(s) -
Clark Catharine Grace,
Kline David D,
Kunze Diana L,
Katz David M,
Hasser Eileen M
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.841.3
Subject(s) - nucleus , neurotrophic factors , brain derived neurotrophic factor , neuroscience , solitary nucleus , medicine , psychology , receptor
Studies from our laboratories have shown that acute administration of BDNF into the nTS increases mean arterial pressure (MAP), heart rate (HR) and lumbar sympathetic nerve activity (LSNA), whereas blockade of endogenous BDNF decreases these cardiovascular parameters in normotensive animals. We therefore hypothesized that BDNF signaling in the nTS contributes to enhanced sympathoexcitation in hypertension. To address this possibility we examined cardiovascular responses to bilateral nTS microinjections of a BDNF scavenging antibody (AntiBDNF; 10μg/ml; 90nl) in the spontaneously hypertensive rat (SHR) model. Wistar‐Kyoto (WKY) and Sprague Dawley (SD) rats served as controls. Acute blockade of endogenous BDNF decreased MAP, HR and SNA in SHR and WKY and SD controls; however the decrease was significantly greater in the hypertensive animals (MAP: SHR Δ −51.8 ± 4.9 mmHg vs. WKY Δ −11.0 ± 1.0, vs. SD Δ −11.8 ± 1.4; HR: SHR Δ −61.3 ± 10.6 bpm vs. WKY Δ −24.8 ± 1.7 bpm vs. SD Δ −20.1 ± 3.3 bpm; LSNA: SHR % Δ −45.1 ± 5.0 vs. WKY Δ −22.4 ± 3.0 vs. SD Δ −29.6 ± 0.5). Similarly, inhibition of the BDNF receptor TrkB by bilateral injection of the tyrosine kinase inhibitor K252a (20μM; 90nl) also decreased MAP and LSNA to a greater extent in the SHR (MAP: SHR Δ −37.4 ± 5.2 mmHg vs. WKY Δ −10.7 ± 1.9; LSNA: SHR % Δ −43.7 ± 5.3 vs. WKY Δ −20.3 ± 2.1). These data suggest that BDNF contributes to sympathoexcitation in hypertension. NS057398