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L‐arginine modulates the inflammatory response caused by unilateral ureteral obstruction
Author(s) -
Gomes Guiomar Nascimento,
Pletiskaitz Thais Fonseca,
Costa Matheus Correa,
Camara Niels Olsen,
Hirata Aparecida Emiko
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.839.5
The aim of this study was to evaluate the effect of L‐arginine (Arg) treatment on the inflammatory response after unilateral ureteral obstruction. Male Wistar rats (12 weeks old) were submitted to ligation of the left ureter (UO). Experimental groups were: control‐sham operated: C; obstructed: UO; control treated with Arg: CA; obstructed treated with Arg: UOA. Groups CA and UOA received as drinking water a solution containing arginine 1% and sucrose 1%, from 24 hours before surgery until 14 days after, when animals were evaluated; Groups C and UO received 1% sucrose solution. We evaluated: macrophage infiltration (immunohistochemistry), MCP1 expression (RT‐PCR), interleukin 6 (IL6) and 10 (IL10) expressions (RT‐PCR and/or Western blotting). Fourteen days after UO we observed an increased macrophage infiltration in UO kidneys. Increased expression of MCP1 and IL6 were also observed in UO kidneys. Arg supplementation reduced macrophage infiltration and the expressions of MCP1 and IL6 in UOA kidneys. IL10, an anti‐inflammatory cytokine, presented a significant increase in UOA kidneys, suggesting that this mediator could contribute to the modulation caused by Arg treatment. Our data suggest that Arg treatment reduces the expression of pro‐inflammatory factors in experimental ureteral obstruction and through this mechanism modulates the inflammatory response and possibly delays kidney damage. Supported by FAPESP, CAPES, CNPq.