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Effect Of Castration On PPADS Modulation of Portal Venous Responses To Splanchnic Nerve Stimulation
Author(s) -
Martin Douglas S,
Kjellsen Barton
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.837.4
Our previous work suggested that castration attenuated the response of the splanchnic vasculature to sympathetic nerve stimulation. The current study tested the hypothesis that ATP contributes to the effect of castration on mesenteric arterial and portal venous responses to splanchnic nerve stimulation. Spontaneously hypertensive rats (SHR) were subjected to sham operation or castration at 5 weeks of age. At 13‐20 weeks of age the rats were anesthetized with urethane/chloralose, instrumented for splanchnic nerve stimulation and for recording of mesenteric arterial and portal venous blood flow and arterial pressure. Frequency response curves (1–15 Hz) were constructed before and after treatment with pyridoxal‐phosphate‐6‐azophenyl‐2′,4′‐disulfonate ( PPADS; 20 mg/kg), an ATP P2X receptor antagonist. Splanchnic nerve stimulation caused frequency dependent decreases in mesenteric arterial and portal venous blood flow. These responses were attenuated in castrated SHR. Treatment with PPADS caused a similar rightward displacement of the frequency response curves in both sham operated and castrated SHR. Collectively, these data suggest that ATP contributes to the mesenteric arterial and portal venous responses to splanchnic nerve stimulation. However endogenous androgens only modestly impact ATP's contribution. Supported by NIH #2RO1HL63053‐ARRA (DM).