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Sex chromosome‐independent ovarian effects on angiotensin converting enzyme (ACE) activity in adipose tissue
Author(s) -
Bajaj Bilkish,
Liu Jun,
Ji Hong,
Zheng Wei,
Wu Xie,
Sandberg Kathryn
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.837.10
Adipose tissue contains the dicarboxylase ACE enzyme, which is implicated in obesity and associated metabolic disease. Sex differences exist in ACE regulation in various target tissues though little is known regarding adipose tissue. We used the four core genotypes (FCG) mouse model in which gonadal sex (ovaries vs testes) is separated from the sex chromosome complement (SCC) enabling comparisons among XX and XY gonadal females (F) and XX and XY gonadal males (M). Adipose ACE activity was greater in the male regardless of the SCC [RFU/min/μg: Intact‐XX‐F, 9.22±1.40; Intact‐XY‐F, 5.55±0.58; Intact‐XX‐M, 26.92±3.90; Intact‐XY‐M, 21.83±0.94; n=6‐5/group; p<0.05, F vs M, by 2‐way ANOVA]. ACE activity was increased in gonadectomized (GDX) females regardless of the SCC whereas no effect of GDX was observed in the males [RFU/min/μg: GDX‐XX‐F, 7.83±0.78; GDX‐XY‐F, 8.29±1.19; GDX‐XX‐M, 10.30±1.10; GDX‐XY‐M, 15.80±3.48; n=6/group]. These findings suggest sex differences in adipose ACE activity in intact mice are due in part to the ovarian hormone milieu and not to the testicular milieu or to differences in sex chromosome dosage (2X vs 1X; 0Y vs 1Y). Ovarian hormone regulation of adipose ACE has particular implications for obesity and associated metabolic disease in women since the ovarian hormone milieu changes radically during puberty, pregnancy and menopause (Supported by R01‐AG19291).