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Changes in citrate transporters in placenta during gestation
Author(s) -
HeringSmith Kathleen S,
Mao Weibo,
Verlander Jill,
Hamm Lee
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.836.13
Subject(s) - placenta , gestation , transporter , obstetrics , pregnancy , medicine , chemistry , biology , fetus , biochemistry , genetics , gene
Placenta (Pl) express a number of transporters of metabolic substrates, including NaDC3 (Na dicarboxylate transporter 3) which was initially cloned from human Pl. We have previously demonstrated a calcium (Ca) sensitive dicarboxylate transport process (citrate and succinate) in OK kidney proximal tubule cells and in the human Pl cell line JAR. Also we demonstrated that JAR contains both NaDC1 and 3, via immunobloting and staining. NaDC1 and 3 are respectively low and high affinity dicarboxylate transporters most studied on the apical and basolateral membranes of renal proximal tubule and intestinal cells. To determine the regulation of NaDC3 and NaDC1 in Pl during gestation, we compared Pl obtained from rats at gestation of 13 days (13d) and 19 days (19d). By immunohisotochemistry, both NaDC1 and NaDC3 were present in rat Pl epithelial cells at both 13d and 19d. For NaDC1, there was a clear increase in the intensity of staining at 19d. For NaDC3, changes in the pattern of staining were not evident. In contrast, by immunoblot of Pl (containing all cell types), NaDC3 decreased (by 27%, p< 0.05) at 19d; NaDC1 modestly increased (by 15%, p< 0.05) at 19d. Since the Pl calcifies with increasing gestational age in pregnancy, and since citrate complexes Ca modulating free Ca available for tissue calcification, these findings raise the possibility that citrate transporters in the Pl regulate its calcification.