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Ovariectomy in rats is associated with increased renal aquaporin‐2, ‐3, –1 and Na,K‐ATPase expression and decreased urine output
Author(s) -
Norregaard Rikke,
Sardeli Chrysanthi,
Kwon TaeHwan,
Li Chunling,
Wang Guixian,
Nielsen Søren,
Frøkiær Jørgen
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.835.2
Female sex hormones are thought to be directly involved in the regulation of renal water handling and solute transport. We hypothesized that dysregulation of renal AQPs is associated with changes in renal water handling after ovariectomy (OVX). Seven and 14 days after OVX the expression of AQP2, ‐3, ‐1 and Na,K‐ATPase were examined in the inner medulla (IM) or whole kidney (WK) by semiquantitative immunoblotting and immunohistochemstry. Semiquantitative immunoblotting revealed a persistent and significant increase in AQP2 and phospho‐Ser256‐AQP2 expression in IM of kidneys from OVX rats at day 7 and 14. Immunohistochemistry demontrated marked labeling of AQP2 in the principal cell of the colleting duct in IM from OVX rats. Consistently, plasma vasopressin levels were significantly increased 14 days after OVX (1.5 ± 0.3 vs. 0.7 ± 0.1 pg/ml in sham operated control). Urine output and water intake were significantly reduced 14 days after OVX. Moreover, AQP3 expression was increased in IM 14 days after OVX. AQP1 and Na,K‐ATPase expression were also significantly increased in WK 14 days after OVX. In conclusion, upregulation of renal AQP2, ‐3, ‐1 and Na,K‐ATPase may play an important role in the increased urinary concentration and decreased urine output after OVX, and hence these could contribute to the water retention associated with altered levels of female sex hormones.