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The effect of inosine and allupurinol administration on xanthine oxidoreductase activity in broilers
Author(s) -
Settle Tabatha Lynn,
Carro Maria,
Falkenstein Elizabeth,
Klandorf Hillar
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.831.17
Xanthine oxidoreductase (XOR) has two forms in the avian, xanthine dehydrogenase (XD) and xanthine oxidase (XO). The purpose of this study was to determine the effects of allopurinol and inosine on plasma uric acid and XOR activity in liver and kidney tissue. Cobb x Cobb (n=15; 5weeks old) were separated into three treatments (n=5); control (CON), INO (inosine at .6M/kg feed), or INOAL (INO and allopurinol 50mg/kg body weight). Birds remained on these treatments for a total of 6 days. Plasma uric acid (PUA) was lower (p< 0.001) in the INOAL group on day 3 as compared to INO. INOAL birds had significantly reduced (p=0.006) kidney uric acid concentrations as compared to birds in the INO treatment. INOAL birds had a significantly lower (p=0.005) uric acid concentration in the liver. There was a significant decrease (p=0.005) in XOR activity of the liver in the INOAL birds. This is indicative of inhibition by allopurinol on XOR activity as compared to control birds. In addition, the reduced uric acid concentration in the liver suggests that there is a residual effect of allopurinol, which potentially results in lowered antioxidant activity, thereby causing an increase in oxidative stress within this tissue.