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Mesenchymal stem cells prevent the blood pressure increase in renovascular hypertension
Author(s) -
OliveiraSales Elizabeth B,
Maquigussa Edgar,
Semedo Patricia,
Câmara Niels OS,
Bergamaschi Cassia T,
Boim Mirian A,
Campos Ruy R
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.826.8
The mechanisms responsible for development of renovascular hypertension (2K‐1C) are still on debate and remain undefined; and thus, alternative therapies are needed. We investigated the effects of mesenchymal stem cells (MSCs) on blood pressure and renal function of 2K‐1C rats. MSCs were obtained from the tibias and femurs of male Wistar rats and expanded for 7–15 days. MSCs were characterized by differentiation and immunophenotyping assays. Adipocyte and osteocyte differentiation confirmed the MSC isolation. Fluorescently tagged MSCs were injected intravenously in rats in which the left renal artery was partially occluded 3 weeks earlier. The systolic blood pressure (SBP) was monitored using tail cuff recording before and after the treatment for 6 weeks. The renal function was evaluated by the determination of plasma and urinary creatinine, proteinuria, urinary excretion of sodium and potassium after 3 weeks of treatment. MSCs had no detectable effects in control rats but prevented the increase of SBP in 2K‐1C. After 6 weeks of clipping, 2K‐1C rats showed significantly increase of SBP compare to control group (224 ± 8 vs 102 ± 1 mmHg, p<0,05). However, the 2K‐1C‐treated rats maintained the SBP (173 ± 6 mmHg) after 3 weeks of treatment. Therefore, there was a significantly reduction in SBP by 22% when compared 2K‐1C to 2K‐1C‐treated rats in the 6 week. The proteinuria was increased in 2K‐1C compare to control animals (15 ± 3 vs 80 ± 19 mg/24h, p< 0,05) and was reduced after treatment (48 ± 8 mg/24h, p< 0,05). The others renal functional parameters unchanged in all groups. Thus, these results suggested that MSCs therapy in the 2K‐1C model can prevents the increase of arterial blood pressure and improves the proteinuria. Supported by CAPES.