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H2O2 Acutely Upregulates Glutathione Peroxidase and Catalase mRNA levels in Cardiac Progenitor Cells
Author(s) -
Pendergrass Karl Dean,
Rafferty Kathryn,
Davis Michael E.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.826.3
Hydrogen peroxide (H 2 O 2 ) is increased in the left ventricle following myocardial infarction (MI). Cardiac progenitor cells (CPC) reside in multiple locations in the heart and may potentially form new cardiomyocytes. CPC migration to damaged areas after MI may provide therapeutic regenerative benefit, aside from transplantation. We previously demonstrated that H 2 O 2 inhibited CPC migration. In the present studies, we sought to determine the regulation of antioxidative enzyme expression in differentiating CPCs after H 2 O 2 treatment. CPCs were isolated and cultured with leukemia inhibitory factor (LIF) to retain their stem‐like qualities. CPCs were allowed to differentiate in the absence of LIF up to 8 days ± H 2 O 2 . Catalase (Cat) and glutathione peroxidase (GPx) were both acutely elevated by Day 5 with 1 μM H 2 O 2 compared to Day 0 (Cat: 4.1±1.0 vs GPx: 2.8±0.9 vs Day 0: 1.0±0; p<0.05; N=4). Furthermore, H 2 O 2 caused a significant upregulation of Cat and GPx mRNA transcripts by Day 5 with 1 and 100 μM, respectively, compared to time‐matched controls (1 μM: Cat: 4.1±1.0 vs Ctl: 1.2±0.2; 100 μM: GPx: 3.4±0.4 vs Ctl: 1.0±0.04; p<0.05; N=4). However, control CPC Cat and GPx mRNA levels reached significance by Day 8 compared to Day 0. In conclusion, we demonstrated that CPCs acutely upregulate antioxidative enzyme gene expression after H 2 O 2 treatment, possibly to protect the cells from injury and maintain migratory capabilities.