Premium
Responses of renin and prorenin receptor –(P)RR‐ in the collecting duct of Sprague‐Dawley rats fed a low salt diet
Author(s) -
Gonzalez Alexis A,
Womack Joel P,
Luffman Christina,
Seth Dale M,
Prieto Minolfa C
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.824.3
Renin and its receptor, the prorenin receptor ‐(P)RR‐ in the collecting duct (CD) may provide a pathway for distal nephron Ang I generation allowing further conversion to Ang II. The (P)RR by binding renin or prorenin, increases the catalytic activity of renin and fully activates prorenin. In chronic angiotensin II (Ang II)‐infused hypertensive rats, the increased intrarenal de novo formation of Ang II is associated with up‐regulation of CD renin. The present study tested the hypothesis that activation of the renin angiotensin system by low salt (LS) diet stimulates renin and (P)RR expression in the CD. We examined the mRNA and protein levels of renin and (P)RR primarily in the renal medulla of rats fed a LS diet (0.3% NaCl) for 7 days. Protein and mRNA levels of (P)RR were significantly higher in medullary tissues from LS‐rats as compared to control animals (142 ± 9 vs. 100 ± 22 % and 140 ± 12 vs. 100 ± 9 %, respectively; P<0.05); however, no differences were observed in kidney cortex. These changes were also associated with increased medullary renin mRNA (390 ± 99 vs. 100 ± 12 %) and intrarenal Ang II levels (435 ± 57 vs. 265 ± 12 fmol/g; P<0.05) Thus, in response to a LS diet, there is augmentation of (P)RR in medullary CD cells which may augment local Ang II levels.