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20‐HETE and CYP 4A isoforms in a model of postmenopausal hypertension
Author(s) -
Moulana Mohadetheh,
Zhang Huimin,
Yanes Licy,
Romero Damian,
Fan Fan,
Purser Chris,
Baker Robert,
Roman Richard,
Reckelhoff Jane
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.822.14
Blood pressure increases in women after menopause. The mechanisms responsible have not been elucidated. The present study was performed to test the hypothesis that 20‐HETE levels and expression of the cytochrome P450 (CYP) 4A ω‐hydroxylases are different in young female (YF, aged 3 mos, n=5–6) and postmenopausal (PMR, 16 mos, n=5–6) spontaneously hypertensive rats. 20‐HETE was measured by liquid chromatography‐mass spectroscopy; renal cortical mRNA expression of CYP4A isoforms were measured by real time RT‐PCR; and CYP 4A protein was measured by western blot. The renal production of 20‐HETE was 2 fold higher in YF than PMR (101.5±5.2 vs 44.4±2.8 pmol/min/mg; p<0.001). There was no difference in production of 20‐HETE in renal or cerebral blood vessels. Renal cortical mRNA expression of CYP4A2 was increased 2 fold (n=8/grp; p<0.01), and CYP4A8 mRNA expression was decreased by 50% (n=8/grp; p<0.01) in PMR compared to YF. CYP4A protein expression was decreased by 15% in PMR. The data suggest that renal production of 20‐HETE is reduced in PMR, and that this may contribute to the resetting of pressure‐natriuresis and development of postmenopausal hypertension in PMR.