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Oxidative stress, inflammation and function of aged lymphatic vessels
Author(s) -
Gashev Anatoliy A.,
Thangaswamy Sangeetha,
Bridenbaugh Eric A.,
Nagai Takashi,
Chatterjee Victor
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.821.44
Effective lymph flow is crucial for fluid/macromolecule homeostasis, lipid adsorption and immunity. It is affected by aging through severe alterations of stretch‐/shear‐dependent adaptive reactions of the contracting lymphatic vessels (LV). Oxidative stress is a well‐known risk factor for aging of blood vasculature; however, its role in the development of aging of LV is unknown. In aged LV, superoxide anion levels may be increased by mitochondrial dysfunction and/or NADPH oxidase activation. The potential role of age‐related disturbances in superoxide dismutase (SOD) levels for activity of aged LV remains unknown. In this study, we evaluated the aging‐related changes in SOD isoform quantity by Western blot analysis and localization in LV wall by immunohistochemical (IHC) labeling in adult (9 mo old) and aged (24 mo old) F‐344 rats. We found that the levels of all SOD isoforms (Cu/Zn SOD, MnSOD and ecSOD) decreased differentially in aged LV. These findings correlate with the aging‐related changes in SOD isoform signals determined by IHC, and are consistent with functional, biochemical, and IHC signs of a chronic inflammatory environment in and around of aged lymphatic wall. These findings serve as first steps in investigating of the aging‐induced mechanisms that link oxidative stress, inflammation, and functional disturbances in aged LV. NIH RO1 AG030578.