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Gamma‐tocopherol attenuates long‐term ovine pulmonary dysfunction after burn and smoke inhalation injury
Author(s) -
Sousse Linda Elias,
Yamamoto Yusuke,
Enkhbaatar Perenlei,
Rehberg Sebastian,
Leonard Scott,
Traber Maret,
Cox Robert,
Hawkins Hal,
Traber Lillian,
Herndon David,
Traber Daniel
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.821.37
We have previously reported that airway nebulization of gamma‐tocopherol (g‐T) had a beneficial effect in the ovine model of acute lung injury. We hypothesize that nebulization of g‐T will improve chronic pulmonary function after burn and inhalation injury. Ewes were divided into uninjured, injured with burn and inhalation, and injured with g‐T treatment groups (n=8) and sacrificed after 3 wks. The expression of dimethylarginine dimethylaminohydrolase‐2 (DDAH‐2), which degrades asymmetrical dimethylarginine (ADMA), significantly decreases after injury compared to uninjured sheep. Arginase activity, ornithine animotransferase (OAT), and collagen significantly increase while pulmonary gas exchange and DL CO significantly decrease after injury compared to uninjured animals. Nebulization of g‐T significantly increases DDAH‐2, pulmonary gas exchange and DL CO , and significantly decreases arginase activity, OAT, collagen deposition, and wound healing areas. Smoke‐induced chronic pulmonary dysfunction is mediated through the reactive oxygen species (ROS)/ADMA/arginase pathway and ROS scavengers such as g‐T may be an alternative for management of inhalation injury. FUNDING : NIH GM066312, SHC Grant 8450