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Effects of N‐acetylcysteine and allopurinol on myocardial adiponectin content and adiponectin receptor expression in rats with streptozotocin‐induced diabetes
Author(s) -
Wang Tingting,
Qiao Shigang,
Lei Shaoqing,
Liu Yanan,
Ng Kwok F.J.,
Irwin Michael G.,
Xia Zhengyuan
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.805.4
Hyperglycemia‐induced oxidative stress plays a central role in the development of diabetic myocardial complications. Plasma level of adiponectin (APN), an adipokine with anti‐diabetic and anti‐inflammatory property, is reduced in diabetes. It is unknown whether the cardiac levels of APN and its receptor expression are altered in rats during early stages of diabetes and whether or not these alterations are modified by antioxidants. We studied the effects of antioxidants N‐acetylcysteine (NAC) and/or allopurinol (ALP) on cardiac levels of APN and its receptor expression in rats with streptozotocin (STZ)‐induced diabetes. Control Sprague‐Dawley (C) or STZ‐induced diabetic (D) rats (220±20g) were treated with NAC (1.5g/kg/day) or ALP (100 mg/kg/day) or their combination for four weeks starting at one week after STZ injection. In D rats, both plasma and cardiac APN level at the 4 th week were decreased. APN receptor 2 (AdipoR2) expression was also decreased (P<0.05) but AdipoR1 expression was increased. NAC restored cardiac APN concentration and AdipoR2 expression (P<0.05), but did not affect cardiac AdipoR1 in D rats. ALP had no effect per se, but potentiated the beneficial effect of NAC. It is concluded that NAC can restore the reduction of myocardial APN and enhance cardiac AdipoR2 expression, and that NAC and ALP can confer synergy in restoring myocardial APN and AdipoR2 expression in rats at early stage of STZ‐induced diabetes despite that ALP alone did not affect myocardial APN.