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Synergy Of N‐Acetylcysteine And Allopurinol Improves Systemic Oxygen Delivery In Rats With Streptozotocin‐Induced Diabetes
Author(s) -
Gao Xia,
Xu Yuan,
Xu Bo,
Lei Shaoqing,
Liu Yanan,
Xia Zhengyuan
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.805.2
Hyperglycemia‐induced oxidative stress plays a central role in the development of diabetic complications. In rats with streptozotocin (STZ)‐induced diabetes, the surface density and total surface area of vascular capillaries was reduced which could impair oxygen diffusion to tissues (Diabetologia. 1995;38:413–21.). We hypothesized that treatment with antioxidants N‐acetylcysteine (NAC) and/or allopurinol (ALP) may attenuate lung tissue oxidative damage and improve systemic oxygen saturation (SO2) in rats with STZ‐induced diabetes. Control Sprague‐Dawley (C) or STZ‐induced diabetic (D) rats (220±20g) were treated with NAC (1.5g/kg/day) or ALP (100 mg/kg/day) or their combination for four weeks starting at one week after STZ injection. In D rats, both plasma and lung tissue levels of 15‐F2t‐isoprostane, a specific index of oxidative damage, were increased (P<0.05 vs. C) at the 5 th week, accompanied with significantly reduced arterial oxygen pressure (pO2) and oxygen saturation (SO2) although partial pressure of carbon dioxide did not significant differ between groups. NAC or ALP alone reduced plasma and lung tissue levels of 15‐F2t‐isoprostane but did not improve pO2 or SO2. However, NAC and ALP combination increased pO2 and SO2 (P<0.05 vs. D). It is concluded that NAC and ALP confer synergy in improving systemic oxygen delivery in diabetes.