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Quantification of Fibrotic and Inflammatory Changes in a Bleomycin‐induced Lung Fibrosis Model
Author(s) -
Garrido Rosario,
Tyagi Gaurav,
Adames Violet,
Wolujczyk Alex,
Peng Ruoqi,
Woods John,
Stevenson Christopher,
Odin Marielle
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.793.7
Histopathological assessment of lung fibrosis models includes routine scoring of resulting fibrosis and inflammation. The goal of this study was to develop methods for automatic quantification of collagen deposits and inflammatory infiltration in the lungs of bleomycin‐treated mice, and to compare the automated readouts with the histopathological evaluation. Male C57BL/6 mice were administered bleomycin intra‐tracheally for up to 21 days. Collagen I immunohistochemistry (IHC) was used to visualize the collagen/fibrotic deposits that developed in the lungs after treatment. Markers for macrophages and lymphocytes were used to characterize the inflammatory infiltrate. Using digital slide scanning and whole‐slide image analysis, we generated a computer‐based method to automatically quantify the fibrotic deposits and the inflammatory cells in the lung. These measurements were then compared with the histopathology scores. Using IHC and whole‐slide automated analysis, we were able to quantify dose‐dependent increases in collagen deposition and changes in macrophage/lymphocyte populations after bleomycin administration. Numerical results from the automated analysis correlated with the histopathological findings. Whole slide customized image analysis is a useful method for consistent, high throughput analysis of changes in the mouse lung after bleomycin administration.