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Analysis of phthalocyanine chloroaluminum phthalocyanine tetrasulfonate and chloroaluminum phthalocyanine incorporated into liposomes to photodynamic therapy in Wistar rats colorectal tumors using proliferative marker Ki‐67 and histomorphometry
Author(s) -
Duarte Janaina,
Rodrigues Katia Calligaris,
Ferreira Alexandre Oliveira,
Junior Landulfo Silveira,
Tedesco Antonio Claudio,
Plapler Helio
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.792.21
Phthalocyanines are widely used in photodynamic therapy (PDT), mainly because of their high absorbance coefficient with optimal tissue penetration by light. This work aimed to compare phthalocyanines with different delivery systems in tumoral tissue. The colorectal tumors were induced in 38 Wistar rats by 1,2‐dimethilhydrazine, and treated by PDT, associating with diode laser irradiation (660nm). Previously was done drugs kinetic by fluorescence spectroscopy, which showed 22 and 26 hours after photosensitizer endovenous injection as the best period of tumors irradiation. The samples were distributed in groups from 0 to 72 hours euthanasia after PDT. The histomorphometry has indicated that necrosis area caused by the therapy has only occurred in chloroaluminum phthalocyanine tetrasulfonate (AlPcS 4 ) groups and the largest areas have occurred in the 72 hours. The chloroaluminum phthalocyanine incorporated into liposomes (AlPHCl) have seemed to be unstable in vivo , which may explain the not occurrence of this purpose. The immunohistochemical performed with the cell proliferation marker Ki‐67 has confirmed the histomorphometry findings. The AlPcS 4 has proved to be more efficient in this experimental model, considering there was remaining tumoral tissue, it suggests the PDT as a adjuvant therapy to other conventional techniques in colorectal tumors.