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Endogenous inhibitors. Postnatal growth and regeneration
Author(s) -
Giorgobiani Nana,
Mosidze Giga,
Rusishvili Levan,
Tavdishvili Elena,
Cherkezia Elena,
Kharadze Ephemia
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.791.9
The goal of the present study was to determine the role of thermostable proteins (TSP) of liver and heart in postnatal growth and regeneration of hepatocytes and cardiomyocytes. Liver and heart tissues of newborn and adult rats were used. Methods included partial hepatectomy, experimental myocardial infarction, alcohol sedimentation of tissue homogenate, native electrophoresis in polyacrylamide gel, and microscopy. Experiments shown identity of TSP of liver and heart by their protein profile that include components with molecular masses of 12–17 and 45–66 kDa. Daily injection of liver TSP to newborn rats during 30 days inhibited proliferation of both hepatocytes and cardiomyocytes. A TSP inhibiting effect was stronger in liver than in cardiomyocytes treated with heart TSP due to a higher mitotic index (MI) in postnatal hepatocytes. After partial hepatectomy liver regeneration was manifested by mitotic activity in 36–48 hours after the operation but mitotic activity in postoperative animals was significantly lower after a single injection of liver TSP. No inhibiting effect of heart TSP could be detected due to the lack of cardiomyocyte mitoses after myocardial infarction. Our studies revealed identical by its protein profile TSP repressor of cell division in the organs with different intensity of cell proliferation and reproductive growth. (This work was supported by the Georgian National Science Foundation)