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Immunomodulatory potential of different dietary fat qualities in a rat model of obesity
Author(s) -
Watzl Bernhard,
Graf Daniela,
Rüfer Corinna E.,
Seifert Stephanie
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.784.9
Subject(s) - obesity , food science , medicine , endocrinology , chemistry
Aim of the study was to investigate the immunomodulatory potential of diets with different fat qualities in a 10‐week feeding trial using the Zucker rat as an animal model of obesity. Rats (lean/obese phenotype; n=12/group) were fed a diet containing 7 % (w/w) fat using corn oil (n‐6/n‐3: 80:1), canola oil (n‐6/n‐3: 2:1) or lard (n‐6/n‐3: 10:1; 0,1% arachidonic acid). Immune cells were isolated from blood, spleen, mesenteric lymph nodes (MLN) and Peyer's patches (PP). Percentage of CD3+ lymphocytes and CD3‐/CD161+ NK cells, expression of CD69 and cytolytic function of NK cells were assessed by flow cytometry. In addition mitogen‐stimulated secretion of IL‐6, IL‐10 and TNF‐alpha was measured. Statistically significant differences for all immune markers were observed between lean and obese rats. Obese rats fed with lard showed less CD3+ lymphocytes in blood (p=0.034), while TNF‐alpha secretion of PBMC was significantly increased (p=0.010). Obese rats supplemented with canola oil had less NK cells in spleen (p=0.012) without affecting their cytolytic function. Cells isolated from the gut‐associated lymphoid system (GALT) were not affected. In conclusion, a low‐fat diet consisting of lard as the only fat source induced proinflammatory changes within the systemic immune system without affecting the GALT. Supported in part by the German Federal Ministry of Research and Education (0315379G).