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Choline intake during lactation alters maternal and infant growth and the ex vivo response of splenocytes to lipopolysaccharide (LPS)
Author(s) -
Dellschaft Neele,
Goruk Susan,
Curtis Jonathan M,
Jacobs Rene,
Field Catherine
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.784.5
To determine if choline is required in the diet during lactation, immediately after birth, the pups from 26 Sprague‐Dawley dams were randomized to the dams and the dams fed one of 3 high fat (20% w/w) diets differing only in the amount and form of choline: a diet with the recommended 1g choline as bitartrate/kg diet (C, n=11) or phosphatidylcholine (PC, n=8), or a choline‐devoid diet (D, n=7). Dams and pups were sacrificed at 21 d and splenocytes isolated from dams and stimulated in vitro with LPS. All results were compared to the choline salt diet, C. Dams and pups weighed less (P<0.05) when fed D. Spleens from D had a higher % of antigen presenting cells (IgM+, OX62+OX6+, CD68+11+) but cells produced less (30%) IFNγ after LPS. Dams, but not pups, weighed more and had a higher gonadal and perirenal fat mass (P<0.05) when fed PC. Spleens from PC had a higher % activated macrophages (CD284+) and Th2 (CD71+), and total and activated B (CD80+28+, IgG+) cells and a lower % T reg cells (CD25+Fox+) (P<0.05). PC cells produced more (P<0.05) IL‐6 (188%) with LPS. These results suggest 1) that choline is needed in the maternal diet during lactation to ensure maternal and infant growth and to support the immune response to LPS and 2) that providing choline as PC instead of a salt may further promote maternal growth, alter the activation state of immune cells and the ability to produce IL‐6 with LPS. (Supported by Alberta Innovates Biosolutions and NSERC.)