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Neonatal calcium deficiency impacts the activity of mesenchymal stem cells
Author(s) -
Seabolt Brynn,
Trakooljul Nares,
Liu HsiaoChing,
Stahl Chad
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.782.11
Calcium (Ca) deficiency alters the activity of mesenchymal stem cells (MSC) in neonatal pigs. Cells from Ca deficient (Ca‐) pigs expressed greater (3 and 2.5 fold) levels of PPARG and AP2 mRNA, respectively, in response to adipocytic induction. To help elucidate the mechanism responsible for this we examined the expression of specific genes and microRNA (miRNA) during proliferation, at confluence, and after adipocytic induction. While proliferating, Ca‐ MSC had greater expression of LIF and C10orf116 mRNA and greater expression of the miRNA, miR‐26a and let‐7c. At confluence, Ca‐ MSC had greater expression of LIF mRNA and reduced expression of TMSB4 and tended to have reduced expression of GLI1 mRNA. The expression of the miRNA, miR‐26a and let‐7c were also greater in Ca‐ MSC at confluence. After adipocytic induction, the reduced expression of GLI1 mRNA in Ca‐ MSC prior to an increase in PPARG mRNA suggests that Hedgehog signaling may be responsible for the changes in differentiation potential of MSC from Ca‐ pigs. Early life Ca deficiency significantly impacts MSC activity and gene/microRNA expression. Alterations in the activity of MSC could have lifetime consequences for bone health.