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Alterations in STAT signaling gene expression during obesity development in dogs
Author(s) -
Grant Ryan W.,
Vester Boler Brittany M.,
Ridge Tonya K.,
Graves Thomas K.,
Swanson Kelly S.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.778.4
STAT proteins are important mediators of growth factor and cytokine signaling. SOCS family members are induced by cytokine signaling and suppress STAT signaling. The purpose of this study was to analyze expression changes of skeletal muscle genes, previously identified by microarray, over 24 wk of ad libitum feeding. Intact female beagles were fed a commercially available high‐fat diet and randomized to either ad libitum feeding (n=5) or weight maintenance (n=4). Latissimus dorsi muscle was collected and body composition was measured at baseline, 4 wk, 8 wk, 12 wk and 24 wk. Expression of mRNA transcripts was measured by qPCR. Obese dogs had increased (P<0.05) body weight, most of which was a result of increased (P<0.05) body fat mass. STAT2 expression was increased (P<0.05). The SOCS family members CISH and SOCS2 had treatment x time interactions, whereby their expression increased in control dogs at 24 wk, but remained unchanged in the ad libitum treatment. Our results indicate gene expression changes that may impact inflammatory signaling. Funding: Illinois CFAR # CF071‐003‐SEN, UNCF‐Merck Science Initiative.