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EFFECTS OF HYPERLIPIDIC DIET CONSUPTION FROM WEANING TO ADULT RATS, ON RENAL AND VASCULAR FUNCTION
Author(s) -
Fiorino Patricia,
Yokota Rodrigo,
Muller Cynthia R,
Farah Vera,
Fonteles Manasses C
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.778.3
The objective of this study was to evaluate the effect of administration of a high fat diet after weaning, during 8 weeks, in rat renal function. Male Wistar rats (n=8/group) were divided in Control group (CG), commercial rat feeding, and Hyperlipidic group (HG) by addition of 30% unsalted butter in commercial diet to rats during 8 weeks, with starts immediately after weaning. The renal function was evaluated in isolated kidney perfused with Krebs‐Henseleit solution containing 6g of previously dialyzed bovine serum albumin. Renal vascular resistance (RVR), urinary flow (UF) and perfusion pressure (PP), were determined every 10 minutes, for 60 minutes. The systolic blood pressure (SBP) was measured by the tail method on the animals through the use of a sphygmomanometer at the eight week. In the HG group we observed important alterations in the PP (CG: 118± 2.5 vs HG: 142±5.2, mmHg), RVR (CG: 5.1±0.5 vs HG: 7.5±0.9, mmHg./ml.g‐1.min‐1) without measurable alterations in the urinary flow (CG: 0.11±0.008 vs HG: 0.18±0.033, mL.g‐1.min‐1). The SBP was significantly increased in HG (138±3.4, mmHg) when compared to the CG (124±1.7, mmHg). These findings suggest that consumption of hyperlipidic diet after weaning led to early changes in both SBP and vascular renal function, as observed by increased in renal vascular resistance, even with the steady state urinary flow. Financial Support: Mackpesquisa (#1430)