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Characterizing the influence of different sources of omega‐3 polyunsaturated fatty acids on renal function and health in female rats
Author(s) -
Gigliotti Joseph,
Turk Philip,
Benedito Vagner A.,
Livengood Ryan,
Tou Janet C.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.777.3
Subject(s) - polyunsaturated fatty acid , creatinine , renal function , medicine , kidney , endocrinology , albumin , chemistry , blood urea nitrogen , urea , biology , biochemistry , fatty acid
Evidence suggests that renal diseases in rodents may be modulated by dietary omega‐3 polyunsaturated fatty acids (ω‐3 PUFAs). Many sources of ω‐3 PUFAs exist and it is unclear how each influences normal renal tissue. Therefore the objective of this study was to determine how different ω‐3 PUFA sources influence renal health. Young (28 d) female Sprague‐Dawley rats (n=10/group) were individually housed in metabolic cages. Rats were pair‐fed 12% high fat diets consisting of corn (CO), flax (FO), menhaden (MO), krill (KO), salmon (SO), or tuna (TO) oils for 8 weeks, when rats were euthanized and serum and kidneys were collected. Renal mineral content was determined using inductively‐coupled plasma spectrometry. Histological evaluation was performed on H&E stained kidney tissue. Serum measures of kidney function included total protein, albumin, blood urea nitrogen (BUN), creatinine, calcium (Ca), and phosphorus (P) measured by colorimetric reactions. KO‐fed rats had heavier (P<0.001) kidneys compared to rats fed FO and MO. Rats fed KO also had the highest (P=0.003) kidney Ca content and displayed more evidence of renal damage by histology. Despite these differences, serum measures of kidney function were similar among the groups. Results suggest that high ω‐3 PUFA intake as KO may result in structural damage of kidneys in the absence of functional changes. Grant Funding Source : USDA‐NIFA #1004489R