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Near‐Infrared Fluorescence for the Quantification of Visfatin in Human Serum: Association with Clinical Markers of Metabolism in Women
Author(s) -
Clark Nina Michelle,
ArizaNieto Magnolia,
Lujan Marla E
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.775.7
Visfatin is a protein secreted primarily by visceral adipose tissue that is purported to have insulin‐mimetic properties. Visfatin may contribute to the development of metabolic disorders but reports are conflicting. The objective of this study was to develop a protocol for profiling and quantifying levels of visfatin in human serum using near‐infrared (NIR) fluorescence Western blots. Associations between serum visfatin and clinical metabolic markers were also determined. Serum was collected from 44 women of reproductive age following an overnight fast and a 75g oral glucose tolerance test. Body composition was assessed by dual‐energy X‐ray absorpitometry (DXA). Western blots yielded a unique 52 kDa band and detection from 16 to 1000pg fell in a linear range. Visfatin levels demonstrated weak and non‐significant associations with BMI, fasting and 2hr glucose levels, percent truncal fat, total body fat, and lean body mass. In summary, visfatin levels can be reliably detected using NIR western blots. Levels of serum visfatin do not reflect metabolic status in women of reproductive age. This research was sponsored by Cornell University. Grant Funding Source : ASN