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Adipose depot differences in adiponectin and the relationship with macrophage infiltration
Author(s) -
Faris Richard J,
WalkerDaniels Jennifer,
Li Jenny,
Jones Douglas E,
Spurlock Michael E
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.775.2
Subject(s) - adipose tissue , adiponectin , infiltration (hvac) , cd14 , medicine , white adipose tissue , endocrinology , gene expression , andrology , biology , flow cytometry , gene , obesity , immunology , biochemistry , insulin resistance , physics , thermodynamics
Fat mass accumulation is linked with infiltration of macrophages (MAC) into adipose tissue as well as decreased serum adiponection (ADN). Thus, the objective of this research was to determine the effect of high fat diets on ADN and MAC infiltration in adipose tissue of Ossabaw pigs fed one of three diets for 30 weeks: a restricted low fat control (LFC), a high fat palm oil (HFP), or a high fat palm oil with n‐3 fatty acids (HFPn3). At the conclusion of the study the subcutaneous (SQ), visceral (VIS), and omental (OM) adipose depots were analyzed by flow cytometry for MAC infiltration (CD203a, CD16, CD14). ADN gene and protein expression were determined in the three depots and in serum. The HFP diet, but not the HFPn3 diet, decreased serum ADN compared to the LFC diet. There was no difference in diet effect in gene or protein expression of ADN. VIS had the highest ADN gene expression, but there were no tissue differences in protein expression. Tissue ADN gene and protein expression did not correlate with any marker of MAC infiltration. Serum ADN only correlated with CD16high positive MAC in VIS (r=−0.56, p=0.01). In conclusion, the HFPn3 diet attenuated the decrease in serum adiponectin, but adiponectin had limited effect on markers of macrophage infiltration. This project was supported by USDA National Research Initiative Grant 2008‐01847 from the USDA National Institute for Food and Agriculture. Grant Funding Source : This project was supported by USDA National Research Initiative Grant 2008‐01847 from the USDA National Institute for Food and Agriculture