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Antioxidant nutrients on lipopolysaccharide‐induced cyclooxygenase‐2 expression in pulmonary epithelial A549 cells
Author(s) -
Lee An Jun,
Choi Sang Yong,
Lim Joo Weon,
Kim Hyeyoung
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.773.8
Acute pneumonia is a most common cause among clinical acute lung injuries. Lipopolysaccharide (LPS), which is a main component of the outer membrane of Gram‐negative bacteria, induces inflammatory responses in respiratory diseases. Cyclooxygenase‐2 (COX‐2) is an inducible form of COX, which plays an important role in the pathogenesis of inflammatory lung diseases. COX‐2 expression is regulated by redox‐sensitive transcription factors including NF‐κB and AP‐1. Reactive oxygen species(ROS) are known as toxic factors in pulmonary inflammation. Present study is purposed to examine whether lipopolysaccharide (LPS) induces COX‐2 expression, which is mediated by ROS production and activation of redox‐sensitive transcription factors in human pulmonary epithelial A549 cells. We examined the effect of antioxidant nutrients ascorbic acid or glutathione on LPS‐induced expression of COX‐2 in A549 cells. As a result, LPS induces expression of COX‐2 and activates NF‐κB and AP‐1 in parallel with ROS production in A549 cells. Antioxidant nutrients inhibited LPS‐induced expression of COX‐2 in A549 cells. In conclusion, antioxidant nutrients may be beneficial for prevention and treatment of pulmonary inflammation induced by Gram‐negative bacterial infection.