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Beneforté™ broccoli with 2–3x phytonutrient glucoraphanin and antioxidant enzyme boosting potential
Author(s) -
Vissavajjhala Prabhakar,
Mero Gene,
Bosch Fransvanden,
Koorevaar Gerard,
Kelly Leo,
James Donald R,
Boddupalli Sekhar
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.773.6
Subject(s) - glucoraphanin , boosting (machine learning) , chemistry , food science , computer science , biology , botany , brassica , machine learning , glucosinolate
Broccoli sulforaphane is demonstrated to be a potent inducer of Phase 2 antioxidant enzymes in cell‐based, animal, and human clinical studies. Beneforté™ broccoli varieties were developed using advanced breeding tools to combine the best attributes of a commercial broccoli with Brassica villosa which has a unique genetic potential to produce increased levels of glucosinolates. Studies over past 5 years in several locations demonstrate that each serving of Beneforté™ broccoli contains 2–3 times glucoraphanin with comparable levels of other nutrients compared to other broccoli varieties grown under similar conditions. Phase 2 enzyme induction of Beneforté™ broccoli was checked using cell based assays. Specifically, broccoli extracts were prepared and HepG2 cells transduced with luciferase under Antioxidant Response Element promoter construct from SABiosciences. Consistent with the 2–3x elevation in glucoraphanin, Beneforté™ broccoli caused 2–3 fold higher ARE‐dependent luciferase expression compared to commercial broccoli extracts. In consumer sensory studies, Beneforté™ has the same great taste as other broccoli varieties and was equally liked when compared in both raw and cooked forms.