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Vitamin E Reduces the Rate of Osteoclastogenesis in Ovarian Hormone Deficiency by Suppressing the Formation of TRAP Positive Cells
Author(s) -
Zadeh Zahra Ezzat,
Soung Do Yu,
Khalil Dania Agha,
Arjmandi Bahram Herman
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.773.2
Subject(s) - ovariectomized rat , osteoclast , medicine , endocrinology , bone marrow , vitamin e , vitamin , bone resorption , chemistry , vitamin d and neurology , basal (medicine) , antioxidant , hormone , diabetes mellitus , biochemistry , receptor
It is generally viewed that with advancing age, humans and other animals including rats experience a gradual increase in the rate of bone resorption. This, in part, may be due to the rise in oxygen‐derived free radical formation. Vitamin E is a strong biological antioxidant which has been shown to slow down the age‐related bone loss in mice. The aim of the present study was to examine the dose‐dependent efficacy of vitamin E in reducing the rate of osteoclastogenesis. Sixty 12‐month old female Sprague‐Dawley rats were either sham‐operated or ovariectomized (ovx; four groups). Rats in one of the ovx groups and the sham group were fed diet with basal level of vitamin E (75mg/kg diet) for 220 days and served as controls. Rats in the remaining three ovx groups were kept on regular diet for the first 120 days and thereafter were given supplemental doses of vitamin E (300, 525, and 750 mg/kg diet). The bone marrow TRAP‐positive multinucleated cells (cells with three or more nuclei) were counted and normalized to1000 total bone marrow cells. The data indicated that osteoclast numbers were significantly higher in ovx control group compared to sham group and this ovx‐induced increase in osteoclast numbers were suppressed by all three supplemental doses of vitamin E, albeit more effectively in ovx group received 300 mg/kg diet vitamin E. These findings suggest that vitamin E may exert its beneficial effects on bone, in part, by reducing the rate of osteoclastogenisis. Grant Funding Source: USDA NRI