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Effects of trans ‐resveratrol supplementation on pAMPK in response to an oral glucose tolerance test and graded exercise test in overweight females
Author(s) -
Hudson Geoffrey Marcus,
Shelmadine Brian D,
Cooke Matthew,
Genovese Joseph,
Greenwood Mike,
Willoughby Darryn S.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.772.10
Subject(s) - overweight , medicine , test (biology) , endocrinology , resveratrol , glucose tolerance test , obesity , pharmacology , biology , insulin resistance , paleontology
Research indicates that resveratrol (RV) can increase glucose uptake via stimulation of the AMPK pathway in rodent models. This study investigated the effects of trans‐RV on pAMPK levels in response to an oral glucose tolerance test (OGTT) and a graded exercise test (GXT). Sixteen women (27.56±5.99 y; 37.94±8.66 kg/m 2 ; 45.86±5.33 % body fat) were randomized to either an RV (n=8) or cellulose placebo (PL, n=8) group and instructed to ingest 250 mg of their respective supplements twice daily for 5 days. On days 6 and 7, participants ingested 500 mg prior to donating a baseline blood and muscle samples. A 75 g glucose solution was then ingested (Day 6) or a GXT (Day 7) performed and a muscle sample was obtained at 1 hr. The pAMPK levels are presented relative to muscle sample weight and analyzed using a repeated measures ANOVA. A trend towards a group × time interaction was observed from Day 1 to Day 6 (PL: −0.90±0.32 vs. RV: +2.10±1.51 μIU/mL‐mg; p = 0.091). A significant group × time interaction was observed following the GXT (PL: +2.32±2.43 vs. RV: −1.70±0.63 μIU/mL‐mg; p = 0.03). No significant time or interaction effects were observed during the OGTT. It is unclear whether RV supplementation increases activation of the AMPK pathway in this population at rest. The greater increase in pAMPK with PL following the GXT could be because the resting levels in the RV group were initially higher than those of the PL group.