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Expression and characterization of thymidylate kinase from Cryptosporidium parvum
Author(s) -
Braunstein Philip,
Hedstrom Lizbeth
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.763.3
Cryptosporidium parvum is an opportunistic pathogen that causes a potentially life‐threatening disease in immunocompromised individuals. This parasite is also a potential bioterrorism agent. There are no effective treatments to cure cryptosporidiosis. Thymidylate kinase is an enzyme essential for nucleoside triphosphate biosynthesis in C. parvum . The C. parvum thymidylate kinase (CpTMPK) was cloned from C. parvum genomic DNA, and expressed in Escherichia coli . Presentation will include purification, and preliminary characterization of CpTMPK. The ultimate goal of this project is to design a high‐throughput screen to identify selective inhibitors of CpTMPK that could be used as lead compounds to develop a drug to treat cryptosporidiosis.