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The involvement of transcription factor, AP‐1 on glutathione synthesis enhanced by caffeic acid, a major component of Perilla frutescens
Author(s) -
Yang SungYong,
Hong ChungOui,
Koo YunChang,
MA YUANYUAN,
Hong YunJin,
Ha YoungMin,
Lee KwangWon
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.722.16
Caffeic acid is a natural polyphenol containing carboxylic acid found in many herbs, and known as an antioxidant in vitro and also in vivo . And also caffeic acid has been reported to have pharmacological effects including antiinflammatory, antimetastatic, antitumorigenic and anticancer. Previous our studies showed water extract of P. frutescens (PLE) protected oxidation‐induced hepatotoxicity, and that caffeic acid isolated from P. frutescens enhanced γ‐glutamylcysteine synthetase (γ‐GCS) activity and glutathione level (GSH). The cellular regulation of GSH level is known to be influenced by nuclear transcription factor, AP‐1. In this study, we investigated the efficacy of caffeic acid and PLE on the synthesis of GSH, and the activation of AP‐1 in human hepatoma (HepG2) cells treated with caffeic acid. Caffeic acid and PLE containing its equivalent level of caffeic acid increased similar GSH concentration and expression of γ‐GCS mRNA in HepG2. And we showed the activation of AP‐1 by caffeic acid with electrophoretic mobility shift assays (EMSA).