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Regulation of De novo Lipogenesis and Lipid Partitioning by Ketogenic Amino Acids in Mice
Author(s) -
Noguchi Yasushi,
Nishikata Natsumi,
Yoshida Chika,
Adachi Yusuke,
Shikata Nahoko
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.722.12
The aim of this study was to assess the effect of dietary ketogenic amino acids (KAA) combined with different dietary lipid sources on hepatic DNL and tissue lipid partitioning in mice. We compared three different KAA‐supplemented diets in which a portion of the dietary protein was replaced by five major essential amino acids (Leu, Ile, Val, Lys and Thr) in palm oil (PO)‐, high‐oleic safflower oil (FO)‐ or soy oil (SO)‐based high‐fat diets. For comparison of the effects of these diets in C57B6 mice, the differential regulation of DNL and dietary lipid partitioning by KAA was assessed via stable isotopic flux analysis. Results Stable isotopic flux analysis revealed high rates of hepatic DNL in high‐PO and high‐FO diets, whereas it was reduced in high‐SO diet. KAA supplementation in high‐PO and high‐FO diets reduced hepatic TG by reducing the DNL of palmitate and the accumulation of dietary oleate. On the other hand, KAA supplementation of the high‐SO diet failed to reduce hepatic DNL and TG. Interestingly, KAA reduced SO‐induced accumulation of hepatic linoleate and enhanced SO‐induced accumulation of dietary oleate. Overall, the reduction of hepatic TG by KAA is dependent on dietary lipid sources and occurs through the modulation of DNL and altered partitioning of dietary lipids.