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In‐vitro tracer‐based investigation on the effect of loquat leaves extract toward glucose metabolism using HepG2 cells
Author(s) -
Wahjudi Paulin N,
Patterson Mary E,
Lu QingYi,
Chen Jian,
Li Weilin,
Lee WN. Paul
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.720.7
Subject(s) - tracer , in vitro , chemistry , carbohydrate metabolism , metabolism , biophysics , biochemistry , biology , physics , nuclear physics
Sesquiterpene isolated from dried loquat leaves has been reported to have anti‐hyperglycemia properties in diabetic animal model. Our study investigated the effect of loquat leaves extract on glucose metabolism in HepG2 cells using [1, 2‐ 13 C 2 ]‐glucose as the tracer. We also compared the effect of the purified sesquiterpene to that of metformin, an antidiabetic drug known for its insulin sensitizing effect. Human hepatoma HepG2 cells were grown in tissue cell culture Dulbecco's modified Eagle's medium containing 50% [1, 2 13 C 2 ]‐glucose in the presence of metformin (1 mM) or sesquiterpene (25 mg/mL). Cells were harvested in 48 hours and mass isotopomers of ribose and deoxyribose were determined. Metformin reduced formation of m1 by G6PDH and increased formation of m2 by TK/TA in ribose/deoxyribose. Using phenotypic phase plane analysis, a significant difference was observed (P< 0.05) in the glycolysis‐pentose cycle pathway between loquat extracts and metformin. Our results suggest that loquat extract acts on different metabolic pathways than those of metformin to achieve its anti‐hyperglycemic effect. (Supported by the UCLA Center of Excellence for Pancreatic Diseases, P01 AT003960 and GCRC, M01‐RR00425)