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Expression of recombinant extremophilic glycosyl hydrolases with biotechnological potential
Author(s) -
RubioMarrero Eva N.,
Cabrera Coral,
Montalvo Rafael
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.688.5
Enzymes are biological catalysts that are essential for different metabolic processes in the cell. Now as day, these processes are being taken as models to fulfill the people needs. The access to energy in our days is considered one of the priorities in our lives, and scientists are working to find out different potential resources for ethanol production. The use of enzymes to produce ethanol derived from biomass has the potential to be a sustainable transportation fuel. This research was focused in using enzymes produced by bacteria that live at high temperatures for the production of bioethanol. To accomplish this, an alpha‐amylase gene of these thermophilic bacteria was cloned and the expression of the recombinant enzymes is being studied. Preliminary constructs are being currently screened for alpha‐amylase activity at 70 °C. The different applications of this research project will lead to reduce the economic and environmental risks, and produce ethanol in a cost‐effective way.