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A COMPARISON OF THE EFFECTS OF PERIAPICAL TOOTH LESIONS ON ADIPOSE TISSUES OF THE RAT
Author(s) -
Zhang Haowei,
Johnson Roger
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.687.3
Our objective was to determine gender‐specific systemic responses to periapical tooth (PA) lesions; specifically on the composition of adipose tissue in the rat. SD rats with gonadectomy(OVX or CAST) and without gonadectomy (N) were studied. In 1/2 of the animals, PA lesions were created by exposing the dental pulps of the maxillary molar teeth to the oral cavity for 28 days (Experimental group, E). 1/2 of the rats were sham‐operated (C). N=16 for each group. 28 days later, the animals were weighed and serum and greater omentum were collected. 1/2 of the tissue was solubilized and 1/2 embedded in paraffin. The tissues were serially sectioned and stained with H&E or immunostained with either anti‐IL‐6, IL‐1β, or TNFα. The cytokine concentrations were determined by ELISA from the solubilized adipose tissues. In addition, 17β estradiol, and testosterone concentrations were determined by ELISA from serum. Data were compared by factorial ANOVA and a post‐hoc Tukey test. At euthanasia, body weight was greater in OVX‐E than in other female groups and lower in CM‐E than in other male groups (p<0.05). Serum IL‐1β, IL‐6 and TNFα concentrations were greater in OVX‐E than either F‐E or F‐C and all male groups (p<0.05). There were no significant differences in the concentrations of these biomarkers between any of the male groups. Immunohistochemistry indicated that cells expressing IL‐1β, IL‐6 and TNFα were within the adipose tissue and not within the blood vessels. The data suggest a synergistic effect of loss of estrogen and presence of PA lesions in females, which results in increased body weight, and enhanced the concentrations of serum and adipose tissue proinflammatory cytokines. These effects were not evident in either N or CAST males with, or without PA lesions. Since PA lesions are evident adjacent to both acute and chronic (unsuccessful endodontic treatment) dental infections, females with dental infections may be more at risk for systemic sequellae than males.