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Aortic Depressor Nerve Ultrastructure in Short Term and Long Term Experimental Diabetes
Author(s) -
Oliveira Fabrício Singaretti,
Nessler Randy Alan,
Salgado Helio Cesar,
Fazan Valéria Paula Sassoli
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.686.9
Subject(s) - term (time) , ultrastructure , diabetes mellitus , medicine , anatomy , endocrinology , physics , quantum mechanics
The aim of this study was to evaluate morphological alterations in the afferent arm of the baroreflex, i.e. the aortic depressor nerve (ADN) of rats, due to short (15 days) and long term (12 weeks) experimental diabetes. Wistar rats received a single intravenous injection of streptozotocin (40mg/Kg) 15 days (N=7) or 12 weeks (N=18) before the experiments, while control animals received vehicle (citrate buffer; N=11 or N=15, respectively). Under anesthesia the ADN was isolated and had the spontaneous activity recorded. Afterwards, the nerves were prepared for transmission electron microscopy studies as reported previously (Fazan et al., 1997; Fazan et al., 2001). The ADN of short term diabetic animals showed clear signs of axonal atrophy for both myelinated and unmyelinated fibers. The ADN of long term diabetic animals also showed axonal atrophy for myelinated fibers, but alterations of the myelin sheath were more evident. Unmyelinated fibers were more preserved in this experimental group. Our results provide morphological support to previous reports of altered function of the baroreflex in short and long term autonomic diabetic neuropathy. Support: CNPq, FAPESP, CAPES and FAEPA. Grant Funding Source : CNPq, CAPES, FAPESP and FAEPA