Premium
The effect of epigenetic factors in heart valve formation
Author(s) -
Kang Hae Jin,
Gahrib Morteza
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.683.6
It is widely known that wall shear stress caused by flow is a major epigenetic stimulus for endothelial cell response in vitro. Klf2a is one of the genes that response to wall shear stress. Klf2a is involved in heart valve formation during embryo development in zebrafish and its expression depends on the presence of reversing blood flow which is an epigenetic factor. To investigate the effect of wall shear stress on valve formation further, the diameter of tail vein of 36~72 hours‐post‐fertilization (hpf) zebrafish emryos is modulated by injecting methylcellulose gel near the vein to increase wall shear stress. Wall shear stress in the tail vein is measured by Particle Image Velocimetry (PIV) technique. The expression of genes that are responding to wall shear stress and involved in valve formation will be investigated using in situ hybridization.