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MOLECULAR ANALYSIS OF NORMAL AND HYPOPLASTIC CHICK EMBRYONIC VENTRICLES
Author(s) -
Pesevski Zivorad,
Almeida Angela,
Mrug Michael,
Fresco Victor,
Barth Jeremy,
Cui Xiangqin,
Krejci Eliska,
Sedmera David
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.683.3
The term hypoplastic left heart syndrome (HLHS) describes a spectrum of cardiac abnormalities characterized by marked hypoplasia of the left ventricle. In a chick model of HLHS, we employed the Affymetrix gene chip technology to gain a better insight into molecular characteristics of embryonic and early fetal myocardial differentiation. RNA isolated from normal left (LV) and right (RV) chick ventricles on ED 6, 8, and 10 was checked for quality using Agilent capillary electrophoresis, and after cDNA synthesis hybridized on the chick whole genome chip. In addition, RNA from ED8 LV and RV of hearts with developed left ventricular hypoplasia after left atrial ligation was treated in the same fashion. Comparisons were made of LV vs. RV at different stages of development, as well as between the control and HLHS group. Interestingly, the number of genes upregulated in the LV was much lower than those higher in the RV. The HLHS group showed delayed molecular differentiation, similar to fetal gene activation in adult heart failure models. This suggests that differentiation of the working myocardium lies more in a restriction of gene expression program, with specific upregulation of select few genes, such as MYH11, MYR8, GBJ6:gap junction protein, beta 6, and others from contractile proteins/myocyte differentiation group of genes, FGF13, NF1 from proliferation/growth factors genes, extracellular matrix genes, metabolism associate gene, and transcription factors. With this data we want to explain roles and involvement of selected genes expressed in the ventricular myocardium at different stages of development as well as their connection with congenital heart disease.