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Further Development Of Tissue Engineered Skeletal Muscle: Repeated Cell Seeding And Increased Mechanical Stretch Augment Early Functional Recovery In A Rodent Muscle Injury Model
Author(s) -
Corona Benjamin T,
Machingal Masood A,
Kesireddy Venu,
Dannahower Ashley,
Koval Christine,
Vadhavkar Manasi,
Zhao Weixin,
Yoo James,
Christ George J
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.678.4
Volumetric muscle loss (VML) injury produces a physiological deficit for which there is no effective clinical treatment. In this study, we determined whether doubling the uniaxial stretching frequency and applying additional MPCs (i.e., 2X‐DS constructs) during bioreactor preconditioning improves functional recovery of LD muscle with VML injury compared to “Generation One” constructs (1X). Morphological assessment before implantation, indicates that 2X‐DS exhibit a greater number of nuclei (2X‐DS vs 1x; 101.2±5.4 vs 49.8±4.2 nuclei/400x field) and multinucleated myotubes (2X‐DS vs 1x; 5.2±0.4 vs 2.0±0.3 myotubes/400x field) compared to 1X. One month after LD VML injury induction and subsequent implantation of 2X‐DS and 1X constructs, muscles repaired with 2X‐DS constructs produced significantly more peak isometric tetanic force than muscles repaired with 1X constructs (2X‐DS vs 1x; 201.3±19.4 vs 145.4±22.4 mN) and injured LD muscles with no repair (88.6±23.9 mN). The results indicate that further optimization of mechanical preconditioning and/or intermittent addition of MPCs during this preconditioning period can promote marked improvement of functional recovery of skeletal muscle with VML injury.