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Real‐time measurement of free radicals in renal veins from exposure to extracorporeal shock wave lithotripsy (ESWL) treatment
Author(s) -
Iames Edward S,
Perkins KerryAnne,
Chen Qian,
Young Lindon H
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.667.10
Clinical studies have suggested ESWL may lead to hypertension and decreased renal function when used as therapy to treat kidney stones. ESWL may cause vascular endothelial dysfunction in the kidney. Endothelial dysfunction is characterized by increased oxidative stress and decreased nitric oxide (NO) bioavailability and serves as a trigger for inflammation‐mediated tissue injury. We tested the hypothesis that ESWL would decrease NO in left renal veins compared to non‐ESWL control rats. NO was directly measured in real‐time by inserting a NO microsensor (100 μm diameter) into the left renal vein in the anesthetized rat. ESWL treatment was administered with a 16 kV shock wave per beat for approximately 13 minutes. 500 shocks were delivered for a period of 60 beats/min and followed by another 500 shocks at 120 beats/min for a total of 1000 shocks. Saline was injected through the tail vein immediately post ESWL or at the same time point in controls. Preliminary data suggests that ESWL‐treated rats (n = 3) significantly reduces NO compared to controls (n = 3) (p < 0.05) during the 30 min post ESWL period. This data supports the hypothesis that ESWL treatment decreases NO bioavailability which may be due to increased oxidative stress. Future studies are aimed at regulating endothelial NO synthase, NADPH oxidase and protein kinase C activities to determine mechanisms to account for oxidative stress. This study is supported by Center for Chronic Disorders of Aging and Department of Pathology/Microbiology/Immunology & Forensic Medicine at PCOM.