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Effects of the diabetes in the metabolism of the catecholamines in rat mesangial cells in culture
Author(s) -
Moreira Roseli Peres,
Cunha Tatiana Souza,
Ronchi Fernanda Aparecida,
Arita Danielle Yuri,
Aragao Danielle Sanches,
Jara Zaira Palomino,
Casarini Dulce Elena
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.666.13
Subject(s) - diabetic nephropathy , medicine , endocrinology , diabetes mellitus , streptozotocin , nephropathy , diabetic retinopathy , metabolism
Diabetes Mellitus (DM) cause a great amount of complications in several organs, developing chronic diseases as diabetic retinopathy, diabetic nephropathy and cardiovascular diseases. This study analyzed the catecholamines (CAs) profile in renal tissue and primary mesangial cells (MC), from normal and diabetic animals. DM was induced in Wistar–Hannover by streptozotocin single tail injection (50mg/kg). The groups ‐ Control (CT), Hyperglycemic (HG) and Insuline Treated Diabetic (TD), were evaluated during 60 days. CAs concentrations were determined by HPLC‐ED. The analysis of CAs in primary MC demonstrated statistically significant increased in the CA levels in HG compared to TD and CT. The CAs renal levels, showed that Norepinephrine is significantly diminished in HG. On the other hand, the other CAs did not present significant difference between the groups. Overall, we can suggest that MC behave in a different manner from global renal tissue and this difference suggests an important role in the production of these hormones that could have a local activity regulating the glomerular filtration. Concluding that in DM we may find a disturb CAs metabolism, besides the renal damage induced directly by high blood glucose levels and these alterations could contribute to the diabetic nephropathy development. Supported by FAPESP, 09/50738‐0.